New Cryopreservation Method Revolutionizes Brain Tissues and Organoids Storage

Breakthrough MEDY method ensures long-term cryopreservation of brain organoids and tissues, maintaining structure and function for diverse biomedical applications.

New Cryopreservation Method Revolutionizes Brain Tissues and Organoids Storage
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 Highlights

  • A novel cryopreservation method, named MEDY (Methylcellulose, Ethylene glycol, DMSO, Y27632), is developed by researchers in China for brain organoids and tissues.
  • MEDY successfully maintains the structural integrity and functional activity of cortical organoids after preservation.
  • The MEDY method is versatile and can be applied to various brain organoids, including those derived from patients, facilitating wide-ranging biomedical applications.

A team of medical researchers from Fudan University in China has developed a groundbreaking cryopreservation method, named MEDY (Methylcellulose, Ethylene glycol, DMSO, Y27632), which promises to revolutionize the storage and use of brain organoids and tissue. This innovative method preserves the structure and functional activity of cortical organoids, making it applicable to a wide range of brain organoids, including those derived from patients.

Brain organoids have become vital tools for modeling brain diseases, but their application has been limited by the challenges of long-term culture, high costs, and storage issues. The new MEDY method, which uses a combination of methylcellulose, ethylene glycol, DMSO, and Y27632, addresses these challenges effectively. MEDY enables the cryopreservation of brain organoids without disrupting their neural cytoarchitecture or functional activity.

The significance of this advancement is profound. MEDY can be applied to multiple brain-region-specific organoids, including the dorsal and ventral forebrain, spinal cord, optic vesicle brain, and epilepsy patient-derived brain organoids. Additionally, MEDY maintains the pathological features of human brain tissue samples after thawing. Transcriptomic analysis has shown that MEDY can protect synaptic function and inhibit apoptosis pathways, ensuring the preservation of neural function.

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This development opens new avenues for biomedical research, medical applications, and drug screening. By enabling the large-scale and reliable storage of diverse neural organoids and living brain tissue, MEDY facilitates extensive research possibilities and practical applications in studying and treating brain diseases. The MEDY method represents a significant leap forward in overcoming the limitations of traditional organoid storage, paving the way for more effective and widespread use of brain organoids in scientific and medical fields.